Fig. 2

Above: Comparison of the structure of ataxin-2 at different levels. The first scheme presents ataxin-2 protein domains (A2D is an additional domain that is in common between ataxin-2 and ataxin-2-like, as reported by Meunier-C et al. 2002 J Biol Chem). The second scheme presents the exons that code for the protein. The third scheme presents the exon structure of ataxin-2. Below: For lanes a – f of the gel picture, each PCR band was sequenced and the shorter bands were shown to be deficient in different exons. Amplification fragments represent exons: A: 2–5 B: 6–9; C: 9–12; D: 12–17; E: 17–22; F: 22–25. RT-PCRs from HeLa RNA were resolved on a 2% agarose-gel, showing the different splice variants. The schematic PCR fragments to the right represent the sequencing results for full length PCR fragments and the shortened variants lacking different exons or exon fragments