Table 1 Sequence of the primers used to perform the PCR for the different fragments of ataxin-2 and GAPDH as loading control
From: New alternative splicing variants of the ATXN2 transcript
RT-PCR | Primer name | Sequence 5’to 3´ | Length (bp) |
|---|---|---|---|
A | A-Forward | TGGGCAGAGGTCGAAACAGTAACA | 24 |
A-Reverse | TGCATCCCAGGGCTCCAGGTC | 21 | |
B | B-Forward | AATGTTCAGACTTTGTTGTGGTA | 23 |
B-Reverse | TCGGGTTGAAATCTGAAGTGTGAG | 24 | |
C | C-Forward | TGAGGGGCACAGCATAAACACT | 22 |
C-Reverse | CGTAGGAGATGCAGCTGGAATAGG | 24 | |
D | D-Forward | GGGGGAACGTGGTCATCAGTGGT | 23 |
D-Reverse | GGTTGCACGCCTGGGCTC | 18 | |
E | E-Forward | TCAGCCAAAGCCTTCTACTACCC | 23 |
E-Reverse | CATGTTGGCTTTGCTGCTGTC | 21 | |
F | F-Forward | CCCAAATTACCATACAACAAGGAG | 24 |
F-Reverse | GATGTGTTCATGACTTTCAAGG | 22 | |
GAPDH | Forward | TTCACCACCATGGAGAAGGC | 20 |
Reverse | GGCATCGACTGTGGTCATGA | 20 |