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Prognostic markers of post-stroke depression (PROMoSD): study protocol of a prospective single-center observational study on raphe hypoechogenicity as a predictor of post-stroke depression
Neurological Research and Practice volume 4, Article number: 59 (2022)
Post-stroke depression (PSD) is an important and frequent non-motor complication after a stroke. As valid prediction of PSD occurrence is still not possible, the unselective use of preventive therapy in stroke patients has risen a questionable risk-to-benefit consideration. Therefore, there is a need to increase the prediction probability of PSD to identify patients at very high risk of a depressive complication who might benefit from preventive therapy. In this context, a brainstem raphe hypoechogenicity (BRH) in transcranial sonography (TCS) has previously been associated with depressive symptoms in a broad spectrum of diseases. BRH might therefore represent a valid maker of vulnerability for depressive symptoms that could be of interest in the risk assessment of PSD occurrence.
In the prognostic markers of post-stroke depression (PROMoSD) study, a prospective, observational, single-center, investigator-initiated study, we aim to include 100 patients with acute ischemic stroke (AIS). Besides data on clinical characteristics and baseline psychiatric assessment, we conduct a TCS examination to identify patients with BRH. The primary outcome is the incidence of PSD three months after inclusion, determined by a blinded investigator according to the fifth version of the Diagnostic and Statistical Manual of Mental Disorders (DSM-V) criteria.
The results of PROMoSD will answer the question of whether screening of BRH after AIS improves the prediction of PSD occurrence. A positive result of this study could have direct consequences on psychiatric support after AIS by streamlining diagnostic and therapeutic algorithms.
Trial registration ClinicalTrials.gov identifier no. NCT05580198.
Stroke represents one of the most common causes of disability and the need for care worldwide . The consequences of a stroke are often severe and affect both motor and non-motor aspects. Post-stroke depression (PSD) is the most common complication after a stroke that contributes substantially to the impairment of the quality of life [2, 3].
Although preventive treatment approaches for PSD have been investigated, these trials have raised with moderate benefits but relevant side effects . It has been postulated that the identification of risk factors for PSD could improve the risk-to-benefit consideration of preventive therapies. In the past, several unspecific PSD risk factors, including stroke severity, previous depressive episodes, and female sex, have emerged as the most common risk factors for developing PSD, but with overall low predictive values . In other diseases, depressive symptoms have been associated with brainstem raphe hypoechogenicity (BRH) which is investigated by transcranial sonography (TCS) [6,7,8,9,10]. Previous data suggest that BR hypoechogenicity may be a general marker of vulnerability for depressive symptoms, but this has never been explored in a prospective study design .
Aim of the trial
There is a need for a better prediction of PSD occurrence. BRH is a potential marker of depression vulnerability. Therefore, the primary aim of the prospective, single-center observational study PROMoSD (prognostic markers of post-stroke-depression) is to investigate whether the presence of a BRH at baseline after an acute ischemic stroke (AIS) predicts the occurrence of a PSD. The secondary aim of this trial is to identify other risk factors that might be associated with an increased risk to develop a PSD three months after an AIS.
Study description and design
PROMoSD is a prospective, observational, single-center, investigator-initiated study which is conducted in the western area of Germany. The local neurological teams of the Department of Neurology of the St. Josef-Hospital Bochum, Ruhr University Bochum, review patients upon admission and will assess eligibility for inclusion in the PROMoSD study. Before inclusion, patients need to give informed consent.
We hypothesize that the presence of a BRH leads to a significant increase in the incidence of PSD. The TCS examination to investigate the presence of a BRH is conducted after inclusion and by investigators who are blinded to the clinical information of the patients. Besides TCS, routine clinical information, common stroke severity scores, serum blood samples, and magnetic resonance imaging data of the brain are collected if available at baseline. The applied set of assessments covers self-reported outcome measures as well as objective scores questioning the presence and severity of depressive symptoms. Initial measures are conducted during the baseline hospital stay. The primary endpoint is the frequency of PSD three months after inclusion which is compared between patients with and without BRH. The Follow-up investigation takes place in a University Hospital for Psychiatry (Fig. 1). The initial and follow-up psychiatric assessment is conducted by blinded investigators. Data collection is carried out according to German data protection law.
Arms and diagnostic intervention
After inclusion, the patients are stratified according to their brainstem raphe status which is determined by the TCS examination. TCS is performed by experienced and board certified (Deutsche Gesellschaft für Ultraschall in der Medizin, DEGUM) investigators who are blinded to any clinical information of the patients. On the other hand, clinical and psychiatric investigators have no access to the TCS data at any time during the study. TCS examination is conducted using a phased array ultrasound system equipped with a 2.5-MHz transducer (Aplio XG Ultrasound System, Toshiba Medicals, Tochigi, Japan). For technical configuration, a penetration depth of 150 mm and a dynamic range of 45–50 dB are chosen. Image brightness and time gain compensation are adapted as needed for each examination. The examination protocol is based on the previously published recommendations for TCS . In detail, using the transtemporal approach, the midbrain and diencephalic examination planes are visualized in the axial section. The grade of BR echogenicity is assessed semi-quantitatively on a three-point scale (0 = raphe structure not visible, 1 = slight and interrupted echogenic raphe structure, 2 = normal echogenicity). In further analysis, grades 0 and 1 are pooled together as hypoechogenic (BRH+: interrupted or absent raphe structure) versus normoechogenic (BRH−: echogenicity of raphe structure is not interrupted and intensity is equal to that of the red nucleus, grade 2) . The sonographic findings are stored to perform a second evaluation and classification of the results by a second investigator. In case of discrepant ratings, a consensus is accomplished.
The primary outcome is the presence of a PSD in the follow-up examination three months after inclusion. PSD is diagnosed by a blinded investigator from the Department of Psychiatry, Psychotherapy and Preventive Medicine, LWL University Hospital, Ruhr University Bochum, Germany. PSD diagnosis is defined according to the fifth version of the Diagnostic and Statistical Manual of Mental Disorders (DSM-V) criteria. Furthermore, patients who receive new medication for anti-depressive indications until follow-up are defined as PSD-positive.
Secondary outcomes are the severity of depressive symptoms measured by the Hamilton rating scale for depression (HRSD), the Beck’s Depression Inventory II (BDI II) as well as the functional outcome measured by the modified Rankin Scale (mRS). Primary and secondary outcomes are assessed at the same time point (Table 1).
Patients admitted to the cooperating Stroke Center following acute ischemic stroke are screened by a physician for eligibility.
The inclusion criteria are:
Patients with ischemic stroke confirmed by brain imaging (either computer tomography or magnetic resonance imaging)
Onset of AIS symptoms within the past 14 days
Age ≥ 18 years
Sufficient German language skills
Declaration of consent
The exclusion criteria are:
No transtemporal bone window for TCS examination
Sample size estimation
We determined that a sample of 100 patients would provide the study with a power of 80% to detect an absolute relative difference of 30% in PSD occurrence with a two-sided alpha level of 0.05 based on the assumption of a 20% prevalence rate of BR hypoechogenicity in a non-depressed population in Germany.
The study was initiated by the Department of Neurology of the St. Josef-Hospital Bochum in cooperation with the Department of Psychiatry, Psychotherapy and Preventive Medicine of the LWL University Hospital Bochum. The study is funded by the Ruhr University Bochum (FoRUM grant K136-20). The study was approved by the local University ethics committee of the Ruhr University Bochum, Germany (approval no. 20-6862). To ensure compliance with the rules of good clinical practice, this study is being monitored by a steering committee (Prof. Christos Krogias [Chair], Department of Neurology, St. Josef-Hospital Bochum, Ruhr University Bochum, Germany; Prof. Georg Juckel, Department of Psychiatry, Psychotherapy and Preventive Medicine, LWL University Hospital, Ruhr University Bochum, Germany; Prof. Georgios Tsivgoulis, Second Department of Neurology, School of Medicine, 'Attikon' University Hospital, National and Kapodistrian University of Athens, Greece, and Department of Neurology, The University of Tennessee Health Science Center, Memphis, TN, USA; Prof. Uwe Walter, Department of Neurology, Rostock University, Germany; Prof. Aristeidis Katsanos, Division of Neurology, McMaster University/Population Health Research Institute, Hamilton, Ontario, Canada).
PSD is probably the most important non-motor complication after a stroke with limited prediction accuracy . Therefore, the PROMoSD study aims to improve PSD prediction by screening for BRH early after an AIS. Current evidence suggests that a BRH investigated by TCS is associated with depressive symptoms and might be a marker of vulnerability for depressive disorders. TCS by its nature is a non-invasive, cost-effective, and feasible diagnostic tool with broad availability and bedside opportunity which makes it very interesting for a wide application. A positive result of this study could have direct consequences for psychiatric support after AIS. Furthermore, it could create the basis for preventive BRH-related therapy trials with the possibility to streamline therapeutic algorithms.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Acute ischemic stroke
Brainstem raphe hypoechogenicity
Fifth version of the Diagnostic and Statistical Manual of Mental Disorders
Hamilton rating scale for depression
- BDI II:
Beck’s Depression Inventory II
Modified Rankin Scale
GBD 2016 DALYs and HALE Collaborators. (2017). Global, regional, and national disability-adjusted life-years (DALYs) for 333 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990–2016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet (London, England), 390(10100), 1260–1344. https://doi.org/10.1016/S0140-6736(17)32130-X
Ayerbe, L., Ayis, S., Wolfe, C. D., & Rudd, A. G. (2013). Natural history, predictors and outcomes of depression after stroke: Systematic review and meta-analysis. The British Journal of Psychiatry: The Journal of Mental Science, 202(1), 14–21. https://doi.org/10.1192/bjp.bp.111.107664
Hackett, M. L., & Pickles, K. (2014). Part I: Frequency of depression after stroke: An updated systematic review and meta-analysis of observational studies. International Journal of Stroke: Official Journal of the International Stroke Society, 9(8), 1017–1025. https://doi.org/10.1111/ijs.12357
Richter, D., Charles James, J., Ebert, A., Katsanos, A. H., Mazul-Wach, L., Ruland, Q., Gold, R., Juckel, G., & Krogias, C. (2021). Selective serotonin reuptake inhibitors for the prevention of post-stroke depression: A systematic review and meta-analysis. Journal of Clinical Medicine, 10(24), 5912. https://doi.org/10.3390/jcm10245912
Das, J., & GK, R. (2018). Post stroke depression: The sequelae of cerebral stroke. Neuroscience and Biobehavioral Reviews, 90, 104–114. https://doi.org/10.1016/j.neubiorev.2018.04.005
Krogias, C., Strassburger, K., Eyding, J., Gold, R., Norra, C., Juckel, G., Saft, C., & Ninphius, D. (2011). Depression in patients with Huntington disease correlates with alterations of the brain stem raphe depicted by transcranial sonography. Journal of Psychiatry & Neuroscience: JPN, 36(3), 187–194. https://doi.org/10.1503/jpn.100067
Richter, D., Woitalla, D., Muhlack, S., Gold, R., Tönges, L., & Krogias, C. (2018). Brainstem raphe alterations in TCS: A biomarker for depression and apathy in Parkinson’s disease patients. Frontiers in Neurology, 9, 645. https://doi.org/10.3389/fneur.2018.00645
Shen, J., Li, D. L., Tan, X. X., Tao, W. W., Xie, C. J., Shi, X. G., & Wang, Y. (2020). A transcranial sonography study of brainstem and its association with depression in idiopathic generalized epilepsy with tonic-clonic seizures. Epilepsy & Behavior: E&B, 102, 106589. https://doi.org/10.1016/j.yebeh.2019.106589
Zhang, Y., Liu, Y., Han, R., Liu, K., & Xing, Y. (2021). Hypoechogenicity of the midbrain raphe detected by transcranial sonography: An imaging biomarker for depression in migraine patients. Therapeutic Advances in Neurological Disorders, 14, 17562864211007708. https://doi.org/10.1177/17562864211007708
Krogias, C., & Walter, U. (2016). Transcranial sonography findings in depression in association with psychiatric and neurologic diseases: a review. Journal of Neuroimaging: Official Journal of the American Society of Neuroimaging, 26(3), 257–263. https://doi.org/10.1111/jon.1232
Richter, D., Schulze, H., James, J. C., Siems, N., Trampe, N., Gold, R., Krogias, C., & Faissner, S. (2022). Hypoechogenicity of brainstem raphe in long-COVID syndrome-less common but independently associated with depressive symptoms: A cross-sectional study. Journal of Neurology, 269(9), 4604–4610. https://doi.org/10.1007/s00415-022-11154-3
Walter, U., & Školoudík, D. (2014). Transcranial sonography (TCS) of brain parenchyma in movement disorders: Quality standards, diagnostic applications and novel technologies. Ultraschall in der Medizin (Stuttgart, Germany: 1980), 354, 322–331. https://doi.org/10.1055/s-0033-1356415
Salter, K., Bhogal, S. K., Foley, N., Jutai, J., & Teasell, R. (2007). The assessment of poststroke depression. Topics in Stroke Rehabilitation, 14(3), 1–24. https://doi.org/10.1310/tsr1403-1
The concept and study design were presented and improved during the 7th European Stroke Organization (ESO) Edinburgh Stroke Research Workshop. Thanks to all participants for your support.
DR receives a FoRUM Grant (K136-20) from the Ruhr University of Bochum to conduct this study.
Ethics approval and consent to participate
The study was approved by the local ethical committee of the Ruhr-University Bochum (approval no. 20-6862). Written informed consent from the patient must be obtained prior to inclusion into this study.
Consent for publication
The consent for publication is integrated into the consent form, which patients must be obtained prior to inclusion into this study.
The authors declare no competing interest.
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Richter, D., Ebert, A., Mazul-Wach, L. et al. Prognostic markers of post-stroke depression (PROMoSD): study protocol of a prospective single-center observational study on raphe hypoechogenicity as a predictor of post-stroke depression. Neurol. Res. Pract. 4, 59 (2022). https://doi.org/10.1186/s42466-022-00225-5
- Post-stroke depression
- Transcranial sonography